A BC D
E FG H
Figure 2. iPSC generation from PD-3 donor (A–D) or Ctrl-1 individual (E–H) fibroblasts. iPSC generation was performed according to the User Guide
for the CytoTune®-iPS Sendai Reprogramming Kit. Briefly, fibroblasts were cultured in DMEM with 20% FBS for 2 days on a 6-well plate coated with
Attachment Factor (A, E). Cells were transduced overnight with the CytoTune®-iPS Sendai Reprogramming Kit at an MOI of 3. At day 7 post-transduction
(B, F) cells were harvested, counted using the Countess® Automated Cell Counter, and plated onto 10 cm MEF culture dishes at 0.5, 1, 2, or 3 x 105
cells/dish. Colonies were picked between days 20 and 26. Representative colonies before picking are shown in C on day 22 or in G on day 26. Picked
colonies were transferred to a 12-well MEF culture plate. Individual colonies were further expanded (D, H), cryopreserved, and characterized.
Instrument highlight—EVOS® XL Imaging System
The cells were monitored and imaged throughout the
reprogramming process using the EVOS® XL Imaging System,
a transmitted light microscope that combines a digital camera,
LCD display, and USB device storage. iPSC colonies were picked
and passaged into new dishes between 20 and 26 days after the
CytoTune® reagents were added to the fibroblasts.
12 Life Technologies | Parkinson's cell model
