Cytogenetic analysis
Over time, chromosomal mutations
in iPSCs can be detrimental to the
health and potential capability of
the cells. Monitoring the stability of
the chromosomes is important to
confirm that the iPSCs are still free of
chromosomal aberrations. For each
iPSC line, cytogenetic analysis was
performed on 20 G-banded metaphase
cells derived from the expanded
clones. The results showed that all
tested clones from all 6 lines displayed
the normal karyotype, confirming
no chromosomal abnormalities.
Representative results are shown in
Figure 3.
A BC
Figure 3. Cytogenetic analysis of iPSCs from PD-2 (A) and PD-3 (B) donors or control individual Ctrl-2 (C). iPSC clones (passage 14 (A),
passage 11 (B), and passage 12 (C)) were cultured in iPSC medium supplemented with KnockOut™ Serum Replacement (KSR) on 6-well MEF
plates for 4 days. Cells were harvested for karyotype analysis. All clones tested demonstrated a normal karyotype. No clonal abnormality was
detected at the G-band level of resolution.
14 Life Technologies | Parkinson's cell model
CULTURE
ENGINEERING
DIFFERENTIATION
CHARACTERIZATION
