Contents
5 What is Parkinson's disease?
5 Collaboration summary
8 iPSC overview
10 Additional mutation search
19 Embryoid body (EB) formation and detection of
three lineage markers
20 Gene expression profiling
21 Detection of transgene expression
11 iPSC generation
22 Analysis using the TaqMan® hPSC Scorecard™
Panel
13 Calculation of reprogramming efficiency
24 Cell line authentication
14 Cytogenetic analysis
25Summary
15 Immunocytochemistry for pluripotency markers
17 Immunostaining and FACS analysis for
pluripotency markers
Find out more at lifetechnologies.com/parkinsons
Human iPSC-derived neurons stained for MAP2 (green) and TH (red), and with Hoechst® stain (blue).
