2 Life Technologies | Genome editing 3 Life Technologies | Genome editing
Table of contents
Introduction to Genome Engineering:
Editing and Modulation
3 Genome engineering workflow
4 The potential for genome engineering
6 Genome engineering solutions
8 Technologies selection table
Genome editing and modulation technologies
10 mirVana
™
miRNA mimics and inhibitors
12 Ambion
®
siRNA
16 Jump-In
™
targeted integration kits
18 Flp-In
™
system
20 GeneArt
®
CRISPR Nuclease Vector
24 GeneArt
®
Custom CRISPR
26 GeneArt
®
Precision TALs
Transfection and analysis
32 Plasmid DNA transfection
36 RNAi transfection
38 GeneArt
®
Genomic Cleavage Detection Assay
Stem cell engineering
40 Engineering stem cells
42 Stem cell engineering resources
Plant genome engineering
44 Precise plant genome editing and
better crop engineering
Custom cell line engineering services
46 TALs stable cell line service
48 CellModel
™
Services stem cell engineering
Genome Engineering Workflow:
Editing and Modulation
At Life Technologies, we have developed a complete suite of products for the
genome engineering workflow, from cell culture and sample preparation to genome
modification, to detection and analysis of known genetic variants. Our featured
technologies include Flp-In
™
and Jump-In
™
protein expression systems, RNAi with
siRNA and miRNA for gene expression modulation, and the newest technologies,
GeneArt
®
Precision TALs and CRISPR (see the selection table on page 8).
Identification: The Ion Proton
®
system is a reliable sequencing platform to identify
and elucidate variants important for the heritability of cancer, as well as Mendelian
and complex disorders. Our bioinformatics package, the Ion Reporter
™
Server System,
is a combined hardware and software solution for automatically analyzing and
annotating human sequence variants.
Detection and analysis: Subsequent to the identification of functional variants and
to create cell models to study these variants, quantitative gene expression studies
using TaqMan
®
and SYBR
®
reagents can be performed on trusted real time PCR
instruments to validate functional variants. Protein expression levels can also be
confirmed using our extensive western analysis platforms and reagents.
Genome engineering and model creation: To further delineate functionality of
variants, our genome engineering tools and services include RNAi, GeneArt
®
CRISPR vectors, GeneArt
®
Precision TALs, and the creation of cell model systems.
Additionally, to assess the cleavage efficiency of genome editing tools at a given
locus, we offer the GeneArt
®
Genomic Cleavage Detection Kit. We even offer a
complete service for engineering your cell of choice to produce custom-designed,
stable cell lines that meet your requirements.
