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FAQ Sections
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CHO-S
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Kit FAQ
Figure 3. Colony PCR on 18 colonies from the EcoRV/PacI cloning. Expected PCR product
size: 750 bp. All 18 colonies carry DNA with the correct insert size.
1–18: Colonies 1 through 18
M: 1 Kb Plus DNA ladder
N: Negative control colony from vector self-ligation. No products expected; faint band is
nonspecific PCR product
N M 1 2 3 4 5 6 7 8 9 N M 10 11 12 13 14 15 16 17 18
850 bp
M 1 2 3 4 5 6 7 8 9 N M 10 11 12 13 14 15 16 17 18 N
Figure 2. Colony PCR on 18 colonies from the BstZ17I/AvrII cloning. Eighteen colonies
carry plasmid DNA with the correct insert size (however, clone 14 was determined to be
incorrect by restriction digestion prior to sequencing).
1–18: Colonies from heavy-chain fragment cloned into pCHO-AvrII/BstZ17I
M: 100 bp DNA ladder
N: Negative control colony from vector self-ligation. No products expected; faint band is
nonspecific PCR product
Expected PCR product size: 530 bp
600 bp
