Issue link: http://life-technologies.uberflip.com/i/330570
15 FAQ Sections What is the risk of contamination (bacterial, mycoplasma, viral) of the Freedom ® pCHO 1.0 Vector? We have compiled the following information that leads us to conclude that the risk of contamination of the stock vector is very low: 1. The LB medium used to grow E. coli is the only source of animal- origin components found in the vector manufacturing workflow. However, the SELECT Peptone 140 (pancreatic digest of casein) used in the formulation of LB comes from New Zealand, Australia, and the United States. These are all countries with very low occurrences of TSE/BSE, and therefore the risk of TSE/BSE contamination from the medium is very low. 2. DNA is prepared using a PureLink ® HiPure plasmid prep kit, which uses a patented anion exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. This level of purity equates to an efficient removal of proteins, nonplasmid DNA, and RNA, as well as any intact microorganisms that may still be present following the lysis step. 3. The final steps in the workflow require the use of alcohol, including 100% isopropanol and 80% ethanol. The use of these alcohols should kill most microorganisms that may have survived previous purification steps. 4. The DNA is prepared using sterile disposable or autoclaved containers, and the final DNA resuspension buffer is also sterile. We strongly recommend that you prepare your DNA (Freedom ® pCHO 1.0 Vector containing your gene(s) of interest) using aseptic techniques and sterile vessels and buffers. Q: A: Life Technologies ™ | Freedom ® CHO-S ® Kit FAQ