17
FAQ Sections
In pCHO 1.0, two genes of interest are driven by two respective
promoters. Is this better in terms of expression than two genes linked
by an internal ribosome entry site and driven by one promoter?
The two promoters in pCHO 1.0 have been specifically engineered to be
distinct in order to avoid any potential issues with recombination, and
to minimize downregulation of the downstream gene despite the fact
that both promoters are strong and constitutive. The use of an internal
ribosome entry site in pCHO 1.0 has not been explored.
What is the exact position of the CMV poly(A) site in the Freedom
®
pCHO Vector?
The AvrII (5765)-BstZ17I (5776) cloning site is located just upstream of
the CMV poly(A) site. The CMV poly(A) site is located at positions 5,773–
6,052. The poly(A) site will still be intact and functional after cutting the
plasmid with AvrII/BstZ17I.
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