21
Is there a preferred signal peptide to use with pCHO 1.0 and should I
optimize my sequence?
• We cannot give any specific recommendations regarding which
signal peptides to use. However, we highly recommend that any
sequence, including genes and signal peptides, be at least evaluated
for optimization via GeneArt
®
services in order to avoid, among
other things, cryptic splice sites, and to improve CHO-specific codon
usage (GeneArt
®
gene optimization services include searches for
other potential issues as well). We know that cryptic splice sites
can severely compromise titer. Users can decide whether or not to
go through with synthesis of the optimized gene after they get the
GeneArt
®
services report.
• Unless it is optimized, avoid using the following signal peptide,
derived from a murine Ig heavy chain locus, in the pCHO vector
(even if it's been successful for secretion in another vector/cell line):
— ATG GGA TGG TCA TGT ATC ATC CTT TTT CTA GTA GCA ACT GCA
ACC GGT GTA CAT TCA
For more detailed information, please see the "Creating Expression
Plasmids for the Freedom
®
CHO-S
®
Kit" section of the User Guide.
Q:
Info:
A:
FAQ Sections
Life Technologies
™
| Freedom
®
CHO-S
®
Kit FAQ
