4 Life Technologies
™
| Required Items
Materials REQUIRED but not included in the kit
Item Recommended vendor Cat. No.
Kanamycin (liquid) Sigma-Aldrich K0254
Restriction enzymes New England Biolabs AvrII: R0174S
BstZ17I: R0594S
EcoRV: R0195S
PacI: R0547S
NruI: R0192S
(Plus any needed to test for insertion/
orientation of gene of interest)
LB Broth Life Technologies 10855021, 10855001
imMedia
™
Growth Medium,
agar, kanamycin
Life Technologies Q611-20
Methotrexate Sigma A6770
Conical tubes Corning (Falcon) 15 mL: 352097
50 mL: 352098
T-flasks, 150 cm
2
Corning 431465
Shake flasks with vented top Corning 125 mL Shake Flasks: 431143
250 mL Shake Flasks: 431144
96-well plates Corning (Falcon) 353072
Falcon Cell Strainer,
40 μm nylon mesh
Corning 352340
Anti-Clumping Agent Life Technologies 20 mL 0010057AE
100 mL 0010057DG
