Q:
A:
34 Life Technologies
™
| Cell Clumping During Freedom
®
CHO-S
®
Workflow
Measure viable cell density and viability AFTER straining cells.
Don't overgrow cells during pre-transfection passage. Keep viable cell
density under 2 x 10
6
viable cells/mL.
Don't use Anti-Clumping Agent prior to transfection.
How do I deal with CHO-S
®
cell clumping?
Clumping with CHO-S
®
cells is to be expected. There are three ways to
minimize cell clumping:
1. Remove clumps with a cell strainer beginning at passage 1 post-thaw.
2. Avoid cell clumps in both sampling and passage of CHO-S
®
cells.
3. Do not allow CHO-S
®
cell densities to exceed 2 x 10
6
viable cells/mL
pre-transfection.
Do not, however, use Anti-Clumping Agent prior to transfection. Later,
inclusion of Anti-Clumping Agent is critical to the success of selection.
Cell Clumping During Freedom
®
CHO-S
®
Workflow
Don't:
Do:
