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Freedom® CHO-S® Kit FAQ

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51 Test transient transfection titers: • If undetectable, do NOT proceed with selection. • If titers are <3 μg/mL, please contact Technical Support (www.lifetechnologies.com). At the website, you can access worldwide telephone and fax numbers to contact Technical Support and Sales facilities. Alternatively, you can: — Email a question directly to Technical Support at: techsupport@lifetech.com — Email a question directly to Gibco ® BioProduction Process Development/PD-Direct ® Services teams at: pd.direct@lifetech.com In Phase I selection (beyond day 7), sample the flasks every 3–4 days; perform a complete medium exchange once a week until the cells show signs of recovery. See Figures 9 and 10 for easy-to-follow flowcharts for both phases of selection. FAQ Sections Life Technologies ™ | Freedom ® CHO-S ® Kit FAQ

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