Q:
A:
57
How long does it take to select for a stable pool? How low does
viability drop?
Phase I selection typically takes 14–18 days, and Phase II selection 7–14
days, for a total of 21–32 days. Viability in Phase I can drop below 10%;
in Phase II, it usually does not drop below 40%.
In this example (see Figure 13), it took a total of 22–32 days to complete
both selection phases. The no-DNA control will drop below 10%
beyond the first week in selection, and will help to distinguish true
recovery during selection. The no-DNA control does not need to be
maintained beyond Phase I selection.
0 2 4 6 8 10 12 14 16
Titer
(mg/L)
Time
(days)
600
800
400
200
0
Neon
®
Stable
Pools
Titers
Neon
2p/1500;
Low-High
Neon
3p/1500;
Low-High
Neon
2p/1600;
Low-High
Neon
3p/1600;
Low-High
0 2 4 6 8 10 12 14 16
Titer
(mg/L)
Time
(days)
FreeStyle
TM
Max
Stable
Pools
Titers
600
800
400
200
0
FSM;
High-High
FSM;
High-Low
FSM;
Low-High
FSM;
Low-Low
FAQ Sections
Figure 11. Titers from Phase II pools transfected
using the Neon
®
Transfection System.
Figure 12. Titers from Phase II pools transfected
using FreeStyle
™
MAX Transfection Reagent.
Life Technologies
™
| Freedom
®
CHO-S
®
Kit FAQ
