68 Life Technologies
™
| Clone Screening
Q:
A:
How should I scale up the clones from 96-well plates to shake flasks?
The flowchart (Figure 16) depicts a suggested workflow for the
major steps in the clone scale-up and screening procedure following
identification of clones to be scaled up from the 96-well plate in
primary screen.
Clone Screening
Q:
A:
What is the typical glutamine and ammonium profile of monoclonal
antibody-expressing CHO-S
®
clones?
Here are typical glutamine and ammonium profiles (Figures 14 and
15) for a clone in a 14-day productivity assessment in CD FortiCHO
™
Medium + 8 mM glutamine + 1:100 Anti-Clumping Agent with glucose
feeding on days 3, 5, and 7 (at 4, 4, and 6 g/L, respectively).
