9
What are your recommendations to clone inserts into the Freedom
®
pCHO
1.0 vector? Should I include Kozak and signal peptide sequences?
1. Use cDNA for your gene(s) of interest.
2. 5'- and 3'-untranslated regions need not be included, as those
functionalities are included in the vector.
3. Include a Kozak sequence, such as GCCACC, immediately preceding
the start codon.
4. A signal peptide is required and if the gene(s) of interest does not have
one, be sure to engineer it into the N-terminus to allow secretion and
easier purification of your desired protein(s).
Q:
A:
FAQ Sections
Life Technologies
™
| Freedom
®
CHO-S
®
Kit FAQ
