28 Life Technologies
™
| Thawing CHO-S
®
Cells
Remove cryovial from water bath while there is still a small ice pellet in
order to prevent overincubation of thawed cells in the presence of the
DMSO cryopreservative.
Do use the correct volume of cell culture media when thawing cells.
Don't submerge the cap or O-ring/cap-vial junction in the water bath.
Don't centrifuge cells upon thawing: removal of DMSO is not required,
as it will be diluted sufficiently upon addition of cells to culture medium.
What is the origin of CHO-S
®
cells?
CHO-S
®
cells are derived from the CHO line established by Dr. Tobey
at the Los Alamos National Laboratoy. CHO-S
®
cells are distinguished
from the commonly used CHO-K1 line based on karyotype
(Chromosome 41:129–144 (1973)), and more recently by sequencing
analysis (Nature Biotechnol 31:759–765 (2013)).
Full citation:
Lewis NE et al. (2013). Genomic landscapes of Chinese hamster
ovary cell lines as revealed by the Cricetulus griseus draft
genome. Nature Biotechnol 31:759–765.
Thawing CHO-S
®
Cells
Q:
A:
Don't:
Do:
