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32 Life Technologies
™
| Cell Clumping During Freedom
®
CHO-S
®
Workflow
Cell clumping creates different issues depending on the stage of
the workflow. We have successfully used a cell strainer at every cell
passage throughout the workflow, both pre-transfection and during
selection, without negative effects. Thus, if in doubt, strain the cells.
However, it is less labor-intensive to use the cell strainer on an
as-needed basis (see video).
Clumping on transfection day:
Cell clumping decreases transfection efficiency, so cell straining is
highly recommended on the day of transfection to ensure that every
cell is sufficiently exposed to transfection reagents.
Clumping during selection:
Cell clumping decreases pool productivity by trapping high-producing
cells in clumps that do not get passaged, so cell straining is critical
during selection to distribute high-producing cells throughout the
culture prior to passage.
Cell Clumping During Freedom
®
CHO-S
®
Workflow
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