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If you cannot clone from the stable pool cell train upon recovery, work
from a frozen vial as follows: thaw the stable pools to be cloned into CD
FortiCHO
™
Medium supplemented with glutamine (Anti-Clumping Agent
is optional; selection pressure is not recommended) and seed for limited
dilution cloning 2–3 days later (without additional pool passage). Pools
should be ≥95% viable before proceeding with limited dilution cloning.
While all steps preceding selection, including pre-transfection passage
and transfection of CHO-S
®
cells, have been done using 130–150 rpm,
we have found more consistent results in selection using 150 rpm.
Whatever rpm setting is used during selection, we recommend keeping
that setting for all subsequent shake flask pool and clone handling
steps, including productivity assessments.
For more detailed information, please see the "Selecting Stable
Transfectants for Protein Expression" section of the User Guide.
Tip:
Info:
FAQ Sections
Life Technologies
™
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®
CHO-S
®
Kit FAQ
