4 Life Technologies | Animal Health
4. Insert a tracheotubus into the trachea and then push the catheter through the tubus
as deep as possible into the lung.
5. Instill sterile sodium chloride solution (or PBS), and aspirate it after 2–3 breaths of
the animal. The amount of solution should not exceed 0.5–0.7 mL per kg of pig.
Sampling technique TNR
1. Restrain the pig with an iron snare. Pay attention to the correct positioning of the
snare, which should always be beyond the first premolars.
2. Clean the nose with a dry piece of paper.
3. Push the catheter through the ventral passage of the nose until the pharynx
is reached.
4. A soon as the pig stops squealing, push the catheter
forward through the wide-open rima glottidis into the
trachea and then into the lower airways. Successfully
reaching the trachea rather than the esophagus is
easily assessed since pigs show spontaneous coughing
when the catheter touches the tracheal epithelium.
5. Instill sterile sodium chloride solution (or PBS), and
aspirate it after 2–3 breaths of the animal. The amount
of solution should not exceed 0.5–0.7 mL per kg of pig.
