Life Technologies

Swine Sampling-Cervical-Swabs-Semen-Urine

Issue link:

Contents of this Issue


Page 2 of 5

3 Life Technologies | Animal Health Preparation Cervical swab In the case of loose housing, selected sows have to be restrained for sampling. Make sure animals are properly restrained in an appropriate fashion by a competent person. Do not take samples in extensively overcrowded pens—pigs may panic and hurt each other or the veterinarian during sampling. • Ensure there is enough light in the work area. • Use a sterile speculum for each sow. • Use swabs of a diameter and length appropriate to the age of the pig (gilt vs. sow). The stem of the swab should be made of plastic, which is less likely to break than a wooden stem (pigs often show defensive movements during sampling). The tip of the swab should be flocked with a synthetic fiber (e.g., Dacron) rather than with cotton, if PCR is the intended diagnostic test: cotton fibers do not release a sufficient amount of swabbed material. • For cultural testing, use swab containers with medium (e.g., Amies). For PCR testing, use swab containers without medium. If both methods are required, take two swabs from each pig and use the two different types of containers. Semen Only boars that have received appropriate training should be selected for sampling. • Make sure that the collection pen is clean and free of extraneous items, which might distract the boar's attention away from the phantom. • Avoid noise, pain, fear, etc., which can negatively influence the boar's behavior for mounting the phantom. Group size Percentage of diseased animals within a group 5% 10% 20% Number of samples (95% confidence level) 100 44 25 13 200 50 26 13 300 53 27 13 750 57 28 13 3,000 58 29 13

Articles in this issue

view archives of Life Technologies - Swine Sampling-Cervical-Swabs-Semen-Urine