Life Technologies

Swine Sampling-Tracheobronchial-Swab

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2 Life Technologies | Animal Health Detection of pathogens—The presence of A. pleuropneumoniae can be confirmed in tonsil scrapings, and the presence of M. hyopneumoniae in tracheobronchial swabs. In either case PCR-based tests can be used to detect pathogen RNA/DNA. A. pleuropneumoniae can also be isolated by cultural techniques. Animal selection Deciding which animals to take samples from depends on the desired outcome: Detection of infection—Select animals with clinical signs. Absence of infection—Select animals with clinical signs considered typical; if there are no symptoms present, take samples from animals selected at random during a walk through the compartment. Keep in mind that for the purposes of monitoring (e.g., SPF status of herd in case of M. hyopneumoniae) other material and methods may be more feasible and reliable. Tracking disease over time (i.e., longitudinal examination)—Take the first samples on day 1 and repeat sampling from the same animals 2 to 4 weeks later. To determine the disease status in different groups (i.e., cross-sectional examination)—Take samples from animals of different ages, e.g., 4, 8, 12, 16, 20, and 24 weeks of age. Diagnostic use

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