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Life Technologies
™
| lifetechnologies.com/parkinsons
When combined, these genetic analysis methodologies
can streamline the TAL-editing process. Table 1 compares
the advantages, limitations, and suggested applications of
each method.
Methodology Advantages Limitations When to use
GeneArt
®
Genomic Cleavage Detection assay • Inexpensive
• Can detect small changes in
homozygous state of DNA—
NHEJ and HDR editing
• Not quantitative
• Limited use if editing heterozygous
loci to homozygous loci
• Triaging colonies from editing
via NHEJ repair
TaqMan
®
SNP Genotyping Assay • Inexpensive
• Fast
• Clearly distinguishes changes in
allele status
• Only detects changes in alleles
that the assay is designed for—
may not detect indels from NHEJ
repair
• Triaging colonies from editing via
homologous recombination
Ion PGM
™
sequencing • Can specifically detect all changes
in a population
• Quantitative results
• Higher cost compared to other
assays
• Longer workflow
• Best used as a secondary assay—
for confirmation and quantitation
of editing in populations identified
from primary screens
Table 1. Comparison of the three genomic analysis methodologies.
Methods
