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29
Sample sequenced Percent mutated
Parental—heterozygous 56
GBA-edited colony pool 23
Edited daughter colony 1
Later passage 1
B
C
0.4
0.4
0.9
1.4
1.9
2.4
0.9 1.4 1.9 2.4
+
Allelic discrimination plot First round
Mutant control
Het control
WT control
GBA +/+ detected
(colony 10)
No template
Legend
Homozygous Allele 1/Allele 1
Heterozygous Allele 1/Allele 2
Allele
2
Allele 1
Homozygous Allele 2/Allele 2
Undetermined
0.4
0.4
0.9
1.4
1.9
2.4
0.9 1.4 1.9 2.4
+
+
+
+
+
+
+
+
+
+
Allelic discrimination plot Second round
Mutant control
Het control
WT control
No template
Legend
Homozygous Allele 1/Allele 1
Heterozygous Allele 1/Allele 2
Allele
2
Allele 1
Homozygous Allele 2/Allele 2
Undetermined
Results
