32
Ordering information
iPSC, MEF, and NSC culture Quantity Supplier Cat. No.
Countess
®
Automated Cell Counter 1 instrument Thermo Fisher Scientific C10227
Geltrex
®
LDEV-free hESC-qualified Reduced Growth Factor Basement Membrane Matrix 1 mL
5 mL
Thermo Fisher Scientific A1413301
A1413302
Essential 8
™
Medium 500 mL Thermo Fisher Scientific A1517001
Thiazovivin 10 mg Tocris Bioscience 3845
StemPro
®
Accutase
®
Cell Dissociation Reagent 100 mL Thermo Fisher Scientific A1110501
D-PBS, Ca
2+
/Mg
2+
free 500 mL Thermo Fisher Scientific 14190144
Basic FGF, Recombinant Human Protein 10 µg Thermo Fisher Scientific
PHG0264
Gibco
®
Mouse Embryonic Fibroblasts (Irradiated) 1 mL Thermo Fisher Scientific S1520100
DMEM with GlutaMAX
™
-I (High Glucose) 500 mL Thermo Fisher Scientific 10569010
Fetal Bovine Serum (FBS), Certified, Heat Inactivated 100 mL Thermo Fisher Scientific
10082139
MEM Non-Essential Amino Acids (NEAA) 100 mL Thermo Fisher Scientific
11140050
GlutaMAX
™
-I Supplement 100 mL Thermo Fisher Scientific 35050061
KnockOut
™
DMEM/F-12 500 mL Thermo Fisher Scientific 12660012
KnockOut
™
Serum Replacement 500 mL Thermo Fisher Scientific 10828028
Gibco
®
PSC Neural Induction Medium 500 mL Thermo Fisher Scientific A1647801
Versene Solution 100 mL Thermo Fisher Scientific
15040066
Colony screening Quantity Supplier Cat. No.
Neon
®
Transfection System 1 each Thermo Fisher Scientific MPK5000
PureLink
®
PCR Purification Kit 50 preps Thermo Fisher Scientific K310001
Zero Blunt
®
TOPO
®
PCR Cloning Kit 20 reactions Thermo Fisher Scientific 450245
BigDye
®
Terminator v3.1 Cycle Sequencing Kit 5,000 reactions Thermo Fisher Scientific 4337457
EVOS
®
XL Imaging System 1 instrument Thermo Fisher Scientific AME3300
E-Gel
®
EX Agarose Gels, 2% 20 gels Thermo Fisher Scientific G402002
Custom TaqMan
®
SNP Genotyping Assay, Human Thermo Fisher Scientific lifetechnologies.com/taqmansnp
Ion PGM
™
System (includes Ion PGM
™
Sequencer and PGM
™
Torrent Server) 1 system Thermo Fisher Scientific 4462921
Genome editing Quantity Supplier Cat. No.
GeneArt
®
Precision TALs Thermo Fisher Scientific lifetechnologies.com/tals
GeneArt
®
Genomic Cleavage Detection Kit 20 reactions Thermo Fisher Scientific A24372
NEW GeneArt
®
CRISPR Nuclease Vector with OFP Reporter Kit 10 reactions Thermo Fisher Scientific A21174
NEW GeneArt
®
CRISPR Nuclease Vector with CD4 Enrichment Kit 10 reactions Thermo Fisher Scientific A21175
32 Life Technologies
™
| Parkinson's cell models
