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Life Technologies
™
| lifetechnologies.com/parkinsons
The Ion Proton
™
Sequencer was used to evaluate potential off-target effects
caused by TAL editing.
Learn more at lifetechnologies.com/proton
Instrument highlight—Ion Proton
™
Sequencer
Screening for off-target modifications in the edited MSA lines
To determine whether TAL-based editing caused any unintended indels at positions
other than the target sites, we sequenced the exomes of the parental and edited
lines using the Ion Proton
™
Sequencer. An Ion Reporter
™
Software analysis of the
sequencing results revealed only six potential off-target indels in the edited MSA
lines, all of which were present in homopolymer regions. Manual inspection of the
sequencing reads at these six positions revealed the indels were likely false positives.
Sanger sequencing was performed and showed that the same sequences were
present in both unedited parental and edited MSA lines. In conclusion, no off-target
indels were detected in the exomes of the two edited MSA lines.
Results
