17 Life Technologies
™
| Genome editing
Ordering information
Product Quantity Cat. No.
GeneArt
®
CRISPR Nuclease Vector: OFP Reporter 10 reactions A21174
GeneArt
®
CRISPR Nuclease Vector: OFP Reporter with Competent Cells
(Combo)
10 reactions A21178
GeneArt
®
CRISPR Nuclease Vector: CD4 Enrichment 10 reactions A21175
GeneArt
®
CRISPR Nuclease Vector: CD4 Enrichment with Competent
Cells (Combo)
10 reactions A21177
Figure 4 . GeneArt
®
CRISPR nuclease vector maps. The vector is prelinearized with 5 bp overhangs for easy
cloning of your double-stranded DNA oligo that encodes a target-specific crRNA. Maps are shown of the
vectors with (A) an OFP reporter and (B) a CD4 reporter. The gRNA, Cas9, and reporter are expressed from the
same vector. Cas9 is directed to the nucleus by nuclear localization signals (NLS1 and NLS2).
TK pA
U6 promoter
CACCG
CAAAA
Pol III
term
tracrRNA
F1 origin
SD4
2A
Cas9 (with NLS1 and NLS2)
P
CMV
Ampicillin
pUC origin
CRISPR Nuclease CD4
Reporter 9,822 bp
B
TK pA
U6 promoter
CACCG
CAAAA
Pol III
term
tracrRNA
F1 origin
OFP
2A
Cas9 (with NLS1 and NLS2)
P
CMV
Ampicillin
pUC origin
CRISPR Nuclease OFP
Reporter 9,219 bp
A
