18 Life Technologies
™
| Genome editing
In the GeneArt
®
CRISPR Nuclease mRNA System,
crRNA and tracrRNA are expressed together as a
single gRNA that mimics the natural crRNA-tracrRNA
hybrid in bacterial systems. The gRNA is encoded by a
custom-synthesized DNA fragment (GeneArt
®
CRISPR
Strings
™
DNA) containing either a U6 or T7 promoter.
GeneArt
®
CRISPR U6 Strings
™
DNA is synthesized with
a U6 promoter and can be directly introduced into mammalian cells for expression of
the gRNA. GeneArt
®
CRISPR T7 Strings
™
DNA is synthesized with a T7 promoter and
can be used as a template for in vitro transcription of the gRNA. Either of these target-
specific gRNAs is cotransfected into cells with GeneArt
®
CRISPR Nuclease mRNA,
which encodes the Cas9 endonuclease (Figure 5). The system is versatile and simple to
use, and changing target specificity only requires a change in the design of the GeneArt
®
CRISPR Strings
™
DNA.
A custom service option is available when a ready-to-use, complete RNA format is
desired. Submit your gene of interest to GeneArtSupport
@
lifetech.com and receive
your target-specific IVT gRNA that is ready for use in your applications.
Find out more at lifetechnologies.com/CRISPRmRNA
GeneArt
®
CRISPR Nuclease mRNA System
High-efficiency CRISPR genome editing tools for multiplex editing
