Issue link: http://life-technologies.uberflip.com/i/438554
28 Life Technologies ™ | Genome editing GeneArt ® TAL effector tools Precise and flexible editing with freedom to innovate Figure 9. Cleavage efficiencies of GeneArt ® PerfectMatch TALs compared to GeneArt ® Precision TALs. To determine the functionality of GeneArt ® PerfectMatch TALs, we have compared the genome cleavage efficiencies of GeneArt ® PerfectMatch TALs to those of GeneArt ® Precision TALs at specific loci using the GeneArt ® Genomic Cleavage Detection Kit (Cat. No. A24372). GeneArt ® PerfectMatch TALs function is equal to or better than GeneArt ® Precision TALs in 293FT cells when targeting sequences of forward and reverse TAL effectors are preceded by different (nonidentical) bases. The red arrowheads point to the cleavage products by GCD enzyme if multiple bands were shown in a GCD assay. TF: TAL effector target site with 5´ T on forward strand; TR: TAL effector target site with 5´ T on reverse strand. CF: TAL effector target site with 5´ C on forward strand; CR: TAL effector target site with 5´ C on reverse strand. GF: TAL effector target site with 5´ G on forward strand; GR: TAL effector target site with 5´ G on reverse strand. AF: TAL effector target site with 5´ A on forward strand; AR: TAL effector target site with 5´ A on reverse strand. HPRT GeneArt ® Precision TAL TF/TR GeneArt ® PerfectMatch TAL CF/AR Negative control GCD Enzyme: + - + - + - + - + - Cleavage efficiency (%) 18.1 19.2 21.6 25.0 IL2 GeneArt ® Precision TAL TF/TR GeneArt ® PerfectMatch TAL CF/TR Negative control GCD Enzyme: + - + - + - + - + - Cleavage efficiency (%) 9.0 12.6 16.4 16.8 RELA GeneArt ® Precision TAL TF/TR GeneArt ® PerfectMatch TAL AF/TR Negative control + - + - + - + - + - 4.9 5.0 16.1 14.3 CCR5 GeneArt ® Precision TAL TF/TR GeneArt ® PerfectMatch TAL GF/TR Negative control + - + - + - + - + - 7.4 10.3 7.4 6.0 ACTB GeneArt ® Precision TAL TF/TR GeneArt ® PerfectMatch TAL GF/CR Negative control + - + - + - + - + - 32.8 37.5 47.6 50.1 IP3R2 GeneArt ® Precision TAL TF/TR GeneArt ® PerfectMatch TAL AF/GR Negative control + - + - + - + - + - 6.6 6.6 13.0 12.9