31 Life Technologies
™
| Genome editing
Doing plant engineering? These resources could help you get started.
Precise plant genome editing and better crop engineering
Generating transgenic plants is key to introducing new
crop traits and carrying out gene studies, both in
discovery and applied settings. The development of
genetically modified plants often requires a complex
design of DNA elements to achieve optimum effects
on expression. Learn more.
GeneArt
®
TALs are ideal for plant research scientists, offering research-use access
with a clear licensing path for commercial use through the Two Blades Foundation.
• Webinar: Watch this webinar in which Dr. Neal Stewart, professor and director at the
Tennessee Plant Research Center, provides novel insights into the potential applications
of synthetic TAL effectors for targeted gene activation of transgenes in plants
• Webinar: Presented by Dr. Jon Chesnut, research fellow and lead scientist at
Thermo Fisher Scientific, this webinar introduces the latest genome editing
technology for plant science researchers
GeneArt
®
PerfectMatch TALs provide custom DNA-
binding proteins designed for accurate DNA targeting
and precise genome editing. Unlike other technologies
that limit the choice of targets, or provide ambiguous
results, GeneArt
®
PerfectMatch TALs help enable
the targeting of any locus in the genome. Previous
versions of GeneArt
®
Precision TALs required a thymine
nucleotide (T) at the 5´ end of each target sequence. The
5´ T constraint limits the fl exibility of TAL effector target
sites in the genome and prevents some specifi c sites
in the genome from being targeted [1]. With our new
GeneArt
®
PerfectMatch TALs, the 5´ T requirement is
removed, making it possible to design a TAL effector pair
for anywhere in the genome, giving you broad fl exibility
in target design. In addition, Thermo Fisher Scientifi c
has recently secured rights to certain intellectual
property around TALs, clarifying a path for you to move
confi dently forward and innovate.
Product details
Transcription activator-like (TAL) effectors are a
widely used technology for precise and effi cient gene
editing in living cells. Native TAL effectors are DNA-
binding proteins produced by plant pathogens of
the genus Xanthomonas. When these bacteria infect
plants, the TAL effector proteins bind to selected
regulatory DNA sequences and directly modulate host
gene expression [2]. The DNA-binding domain of TAL
effectors consists of a variable number of amino acid
repeats, called repeat-variable di-residues (RVDs),
which recognize a single DNA base pair. We provide
GeneArt
®
TALs with special functional domains
(nuclease, activator, or repressor domains) that are
designed to interact with unique DNA targets in large,
complex genomes.
PRODUCT GUIDE GeneArt
®
PerfectMatch TALs
GeneArt
®
PerfectMatch TALs
Precise and extraordinarily fl exible genome editing
Find more data and application examples in our
GeneArt
®
PerfectMatch product guide.
