Issue link: http://life-technologies.uberflip.com/i/438554
44 Life Technologies ™ | Genome editing Nucleic acid transfection techniques for mammalian cells vary widely and include lipid-based transfection and physical methods such as electroporation. Lipofectamine ® transfection reagents are the most cited and trusted in the world because of their superior transfection performance and broad cell spectrum. An overview of our most effective transfection products is shown in Table 2 to help you choose the solution that's right for you. Superior Transfection Reagents Enhanced gene editing outcomes with superior transfection reagents Table 2. Transfection selection guide. More blocks represent higher transfection efficiency into a greater number of cell types Transfection selection guide Table 1. Transfection selection guide—more blocks represent higher transfection efficiency into a greater number of cell types. Transfection product DNA mRNA RNAi Co- delivery* Easy-to-transfect cells Difficult-to- transfect cells Primary cells Stem cells Suspension cells Superior transfection reagents Lipofectamine ® 3000 Transfection Reagent Lipofectamine ® RNAiMAX Transfection Reagent Lipofectamine ® MessengerMAX ™ Transfection Reagent ExpiFectamine ™ 293 Transfection Kit Recommended for Expi293F ™ cells Broad-spectrum transfection reagent Lipofectamine ® 2000 Transfection Reagent Electroporation Neon ® Transfection System In vivo delivery Invivofectamine ® 2.0 reagent In vivo delivery to liver following tail vein injection Transfection is the process by which nucleic acids are introduced into eukaryotic cells. Techniques vary widely and include lipid-based transfection and physical methods such as electroporation. Lipofectamine ® transfection reagents are the most trusted and cited in the scientific literature due to their superior transfection performance and broad cell spectrum. An overview of our most effective transfection products is shown in Table 1 to help you choose the solution that's right for you. 5,000 10,000 15,000 20,000 25,000 30,000 35,000 40,000 45,000 50,000 0 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 2014 Cumulative number of publications citing the use of Lipofectamine ® family of reagents since 1996. 4 *Cotransfection of RNAi vector and siRNA. * Cotransfection of RNAi vector and siRNA