34 Life Technologies
™
| Genome editing
Flp-In
™
system
Efficient integration into a specific genomic location
The Flp-In
™
system allows stable integration and expression of your gene of
interest, to deliver single-copy isogenic cell lines. Flp-In
™
expression involves
introduction of a Flp recombination target (FRT) site into the genome of the
mammalian cell line of choice. An expression vector containing your gene of
interest is then integrated into the genome via Flp recombinase–mediated DNA
recombination at the FRT site.
We offer a wide selection of Flp-In
™
products, including expression vectors and
systems, as well as parental cell lines with a stably integrated FRT site. Designed
for rapid generation of stable cell lines, these products help ensure high-level
expression of your protein of interest from a Flp-In
™
expression vector.
Find out more at lifetechnologies.com/flpin
Gene of interest
+ pOG44
(Flp recombinase)
ATG
SV40
pA
BGH
pA
SV40
pA
FRT FRT P
SV40
P
CMV
goi
IacZ-Zeocin
TM
hygro
pUC ori Amp Amp pUC ori
Flp-In
TM
expression cell line
(hygromycin-resistant, Zeocin
TM
-sensitive)
pcDNA
TM
5/FRT
goi = gene of interest cloned
into pcDNA
TM
5/FRT
Flp-In
TM
Expression
Vector
B
GH
p
A
S
V
4
0
p
A
puc
or
i
A
m
p
i
c
illi
n
P
C
M
V
H
yg
ro
m
yc
i
n
FR
T
Site-specific gene integration tools
Targeted integration of your gene of interest into specific integration
