Life Technologies

36 Life Technologies ™ | Genome editing Gene modulation RNAi tools Transient knockdown of multiple transcripts RNA interference (RNAi) is a specific, potent, and highly successful approach for loss- of-function studies in virtually all eukaryotic organisms. We have developed two types of small RNAs that function in RNAi: short interfering RNA (siRNA) and microRNA (miRNA). We offer products for RNAi analysis in vitro and in vivo, including libraries for high-throughput applications. Your choice of tool depends on your model system, the length of time you require knockdown, and other experimental parameters. Ambion ® siRNA Superior siRNAs for in vitro applications RNAi is the best way to effectively knock down gene expression to study protein function in a wide range of cell types. Traditional RNAi methods for gene knockdown in mammalian cells involve the use of synthetic RNA duplexes consisting of two unmodified 21-mer oligonucleotides annealed together to form small interfering RNAs (siRNAs). Silencer ® Select siRNA products incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemistry, to offer: • High potency—improved siRNA prediction accuracy compared to Silencer ® siRNA (Table 1) • Minimal off-target effects—Locked Nucleic Acid (LNA) chemical modifications reduce off-target effects by up to 90% • Guaranteed—100% guaranteed to silence, for increased confidence in your reagents** • Open access—65,000 siRNA sequences and associated data on PubChem from our Silencer ® Select siRNA library ** siRNA guarantee (a) Silencer ® Select siRNA: Thermo Fisher Scientific guarantees that when you purchase two Silencer ® Select Pre-Designed siRNAs to the same target, then those two siRNAs transfection. Real-time RT-PCR is recommended but not required for this application. Customers must also show sufficient knockdown with a positive control siRNA to demonstrate sequence will be synthesized free of charge. This guarantee does not extend to any replacement product. (b) Stealth RNAi ® : Thermo Fisher Scientific guarantees that when you purchase three Stealth RNAi ® Pre-Designed siRNAs to the same target, then at least two of those three mRNA levels detected 48 hours post-transfection. Real-time RT-PCR is recommended but not required for this application. Customers must also show sufficient knockdown successful, a new Silencer siRNA sequence will be synthesized free of charge. This guarantee does not extend to any replacement product. We also recommend the use of (c) Silencer ® products: Thermo Fisher Scientific guarantees that when you purchase three Silencer ® Pre-Designed siRNAs to the same target, at least two of the siRNAs will conditions described below. If at least two of the three siRNAs do not induce >70% target mRNA knockdown, Thermo Fisher Scientific will provide a one-time replacement days from the date of delivery of the Silencer ® Pre-Designed siRNAs. Optimum transfection efficiency must be confirmed using good laboratory practices and a proven-to-work compared to that of cells transfected with a nontargeting control siRNA, such as Silencer ® Negative Control #1. We recommend TaqMan ® Gene Expression Assays to quantify

• Cover