Life Technologies

Genome modulation and editing

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41 Life Technologies ™ | Genome editing Figure 10. mirVana ™ miRNA inhibitors effectively suppress miRNA in vivo. miR122 or Negative Control #1 mirVana ™ miRNA inhibitors were complexed with Invivofectamine ® 2.0 Reagent and delivered to BALB/c mouse liver via tail vein injection on 3 consecutive days at a dose of 7 mg per kilogram of body weight. Expression of four mRNA targets (AldoA, Hfe2, Slc35a4, and Lass6), natural targets of miR122, were measured in transfected livers of mice injected with miR122 miRNA inhibitor or Negative Control #1 (Neg 1) and livers of mice that were not transfected (NT) using TaqMan ® MicroRNA Assays. This indicates that mirVana ™ miRNA inhibitors are efficiently delivered to the liver with Invivofectamine ® 2.0 Reagent, leading to upregulation of genes naturally suppressed by miR122. 0 50 100 150 200 250 300 350 400 450 miR 122 NT Neg1 miR 122 NT Neg1 miR 122 NT Neg1 miR 122 NT Neg1 AldoA Hfe2 Slc35a4 Lass6 mRNA upregulation % miR122 = miR122 miRNA inhibitor NT = Not transfected Neg1 = Negative Control #1

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