50 Life Technologies
™
| Genome editing
When using genome editing tools to obtain targeted
mutations, it is necessary to determine how efficiently
they cleave the target sequence, particularly prior
to proceeding to the more laborious and expensive
cell line cloning and sequencing processes. The
GeneArt
®
Genomic Cleavage Detection Kit provides a
relatively quick, simple, and reliable assay that allows
the assessment of the cleavage efficiency of genome
editing tools at a given locus (Figure 13). A sample of the edited cell population
is used as a direct PCR template for amplification with primers specific to the
targeted region. The PCR product is then denatured and reannealed to produce
heteroduplex mismatches where double-strand breaks have occurred resulting
in indel introduction. The mismatches are recognized and cleaved by the detection
enzyme. Using gel analysis, this cleavage is both easily detectable and quantifiable.
• Easy—with direct PCR amplification, there's no need for genomic
DNA isolation
• Rapid—5-hour total processing time
• Quantitative—gel band density is directly correlated to target indel
introduction
GeneArt
®
Genomic Cleavage Detection Kit
A quick, simple, and reliable method for detecting and quantifying locus-specific
