54 Life Technologies
™
| Genome editing
GeneArt
®
Genomic Cleavage Selection Kit
A quick method for screening the functionality of nuclease cleavage
The GeneArt
®
Genomic Cleavage Selection Kit is a rapid and reliable tool for
detecting functionality of engineered nucleases in transfected cells as well as
enriching for modified cells. When using engineered nucleases to create double-
stranded breaks in genomic DNA, it is necessary to know whether or not the
designed nucleases are functional. Furthermore, to efficiently screen for modified
cells, a way to enrich for the edited cells is also necessary, particularly if the
engineered nuclease has low efficiency or the cell line used is difficult to transfect.
The GeneArt
®
Genomic Cleavage Selection Kit contains a vector with the orange
fluorescent protein (OFP) gene for a quick visual check of the functionality of the
engineered nuclease. In addition, the reporter genes OFP and CD4 can be used
to enrich for edited cells. It can be used in conjunction with genome editing tools
such as zinc finger nucleases (ZFNs), transcriptional activator-like (TAL) effector
nucleases, and CRISPRs.
• Screen—for functionality of engineered nucleases as early as 24 hours
post-transfection using fluorescence standard microscopy
• Enrich—for modified cells using fluorescence-activated cell sorting (FACS)
or Dynabeads
®
CD4 magnetic beads
Ordering information
Product Quantity Cat. No.
GeneArt
®
Genomic Cleavage Selection Kit 10 reactions A27663
