58 Life Technologies
™
| Genome editing
Ion PGM
™
sequencing
To expedite detection and quantitation of TAL editing events, we developed an
amplicon sequencing method on the Ion PGM
™
System (Figure 17). This method
allows you to generate thousands of sequencing reads from any one sample
and to process up to 96 samples in one sequencing run. With this method, you
are able to determine whether an isolated iPSC colony harbors any cells that
contain a desired mutation, whether that colony contains a clonal or mixed
population of cells, and whether a clonal population of cells was homozygous
or heterozygous for the mutation.
Genome editing analysis tools
