Issue link: http://life-technologies.uberflip.com/i/438554
7 Life Technologies ™ | Genome editing With all the tools and technologies available today, which ones are right for your research? We'd like to help simplify your choice, based on your research needs. Once you determine which technology best suits your needs, you can learn more about the specific tools available in the following sections CRISPR-Cas9 tools TAL effector tools Rapid and efficient editing with multiplexing capabilities Precise and flexible editing; targeting to any gene in any cell, with all-around freedom GeneArt ® CRISPR Nuclease Vector GeneArt ® CRISPR Nuclease mRNA GeneArt ® Precision TALs GeneArt ® PerfectMatch TALs • Easy-to-design genome engineering system • Affordable, ready-to-use mRNA or cloning vectors • Enrichment for hard-to-transfect or difficult-to-modify cell lines (GeneArt ® CRISPR Nuclease Vector) • Multiplexing and screening capable, with easy construction and low cost • Precise technology that recognizes the DNA sequence you specify • Flexible design lets you choose the effectors and Gateway ® cloning–compatible vector that meet your needs • Reliable, because the final clone contains a verified, optimized sequence for improved expression • Fast service delivers your clone, typically within 2 weeks of your order confirmation • Independent of target sequence, with no restrictions for GeneArt ® PerfectMatch TALs • Gene deletion (knockout) • Integration (knock-in) • Gene deletion (knockout) • Downregulation (knockdown) • Integration (knock-in) • Gene activation • Simple and fast design process • Difficult to design (except when using the GeneArt ® TALs online design tool) RNA–DNA Protein–DNA • PAM site (NGG at the end of the 20-bp target sequence) • Active range of spacing needed for activity, no design constraints • Gene function • Gene modulation • Gene tagging • Pathway engineering • Metabolic engineering • Protein production • Gene function • Gene modulation • Gene tagging • Pathway engineering • Metabolic engineering • Protein production • Off-target effects • Inhibited by chromatin structure • Large size for delivery Moderate Low Capable Rarely used • Mammalian • Bacterial • Yeast • Insect • Stem cells • Zebrafish • Mammalian • Bacterial • Yeast • Plants • Insect • Stem cells • Zebrafish