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| Genome editing
Figure3. A CRISPR-Cas9 targeted double-strand break. Cleavage
occurs on both strands, 3 bp upstream of the NGG protospacer-
adjacent motif (PAM) sequence at the 3´ end of the target sequence. The
specificity is supplied by the guide RNA (gRNA), and changing the target
only requires a change in the design of the sequence encoding the guide
RNA. After the gRNA unit has guided the Cas9 nuclease to a specific
genomic locus, the Cas9 protein induces a double-strand break (DSB) at
the specific genomic target sequence.
Watch this video for a quick understanding of how the CRISRP-Cas9 system works.
Bench Tip Video: CRISPR-Cas Genome Editing Technology
