Life Technologies

26 Life Technologies ™ | Genome editing TAL effectors are a widely used technology for precise and efficient gene editing in live cells. The deciphering of the TAL effector "code" led to the engineering of designer TAL effector proteins. We have recently secured rights to certain intellectual property (e.g., patented methods) around TALs, clarifying a path for you to move confidently forward and innovate. GeneArt ® TALs provide custom DNA binding proteins engineered and designed for accurate DNA targeting and precise genome editing. GeneArt ® TALs offer site-specific delivery of nucleases, activators, repressors, chromatin modifiers, genomic labels, and crosslinking molecules (Figure 7). This genome editing technology is known to function in a variety of host systems, including bacteria, yeast, plants, insects, fish, and mammals. Based on your research needs, you can select from our two different formats of TAL effector tools: GeneArt ® Precision TALs or GeneArt ® PerfectMatch TALs (Figure 8). Choose GeneArt ® Precision TALs when working with plants, or if you have no design constraints. Choose GeneArt ® PerfectMatch TALs when you need complete flexibility in target design, removing the 5´ T constraint for targets. GeneArt ® PerfectMatch TALs are derived from GeneArt ® Precision TALs and contain a truncated TAL effector fused to a FokI nuclease domain. GeneArt ® PerfectMatch TALs contain 3 amino acids mutated at the N terminus of the TAL effector of a GeneArt ® Precision TAL. The mutation converts the 5´ T binding motif at the TAL effector N terminus to a universal binding motif (it will bind to any base: A, G, C, or T). GeneArt ® TAL effector tools Precise and flexible editing with freedom to innovate

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