Issue link: http://life-technologies.uberflip.com/i/438554
37 Life Technologies ™ | Genome editing siRNA controls Proper controls are essential to help ensure success in every RNAi experiment. The number and types of controls chosen depend on the ultimate research goal, and we offer positive and negative controls, as well as GeneArt ® optimized gene synthesis for siRNA-resistant genes that can be used in RNAi rescue experiments. For more information, go to lifetechnologies.com/sirnacontrols and lifetechnologies.com/geneartgenesynthesis Table 1. Ambion ® siRNA selection guide. Cost-effective siRNA Good knockdown, low off-target effects Highest knockdown, lowest off-target effects Silencer ® siRNA Stealth RNAi ™ siRNA Silencer ® Select siRNA Potency 100 nM recommended concentration 20 nM recommended concentration 5 nM recommended concentration Efficacy (>70% knockdown) 2 of 3 siRNA guaranteed 2 of 3 siRNA guaranteed 2 of 2 siRNA guaranteed Target specificity Moderate High Highest Coverage Coding RNA Coding RNA Coding and noncoding RNA Target species Human, mouse, rat (use custom tool for other species) siRNAs will silence the target mRNA by 70% or more. To qualify for the guarantee, siRNAs must have been transfected at ≥5 nM and mRNA levels detected 48 hours post- demonstrate transfection efficiency. If the guaranteed level of knockdown is not observed and an appropriate positive control is successful, a new Silencer ® Select siRNA three independent, non-overlapping siRNAs will silence the target mRNA by 70% or more. To qualify for the guarantee, siRNAs must have been transfected at ≥20 nM and knockdown with a positive control siRNA to demonstrate transfection efficiency. If the guaranteed level of knockdown is not observed and an appropriate positive control is of an appropriate negative control, such as one of the three Stealth RNAi ® Negative Controls to normalize message knockdown. will reduce target mRNA levels in cultured cells by 70% or more when measured 48 hours after transfection at 100 nM or higher final siRNA concentration under the replacement of up to three Silencer ® Pre-Designed siRNAs per target at no additional charge. Requests for replacement product must be made within one hundred and eighty (180) proven-to-work siRNA to an endogenous message, such as Ambion ® Silencer ® GAPDH siRNA Control. To assess knockdown, target mRNA levels in treated samples must be quantify mRNA levels.