41 Life Technologies
™
| Genome editing
Figure 10. mirVana
™
miRNA inhibitors effectively suppress miRNA in vivo. miR122 or Negative Control #1 mirVana
™
miRNA inhibitors were complexed with Invivofectamine
®
2.0 Reagent and delivered to BALB/c mouse liver via tail
vein injection on 3 consecutive days at a dose of 7 mg per kilogram of body weight. Expression of four mRNA targets
(AldoA, Hfe2, Slc35a4, and Lass6), natural targets of miR122, were measured in transfected livers of mice injected
with miR122 miRNA inhibitor or Negative Control #1 (Neg 1) and livers of mice that were not transfected (NT) using
TaqMan
®
MicroRNA Assays. This indicates that mirVana
™
miRNA inhibitors are efficiently delivered to the liver with
Invivofectamine
®
2.0 Reagent, leading to upregulation of genes naturally suppressed by miR122.
0
50
100
150
200
250
300
350
400
450
miR
122
NT Neg1 miR
122
NT Neg1 miR
122
NT Neg1 miR
122
NT Neg1
AldoA Hfe2 Slc35a4 Lass6
mRNA
upregulation
%
miR122 = miR122 miRNA inhibitor
NT = Not transfected
Neg1 = Negative Control #1
