Issue link: http://life-technologies.uberflip.com/i/438554
6 Life Technologies ™ | Genome editing Genome modulation and editing technologies Find the right technology for your application Technology class RNAi Site-specific gene integration Requirement Transient knockdown of multiple transcripts Targeted integration of your gene of interest into specific integration sites Products Silencer ® Select siRNA Ambion ® miRNA mimics and inhibitors Jump-In ™ Targeted Integration Kits Flp-In ™ System Benefits • High-potency, transient knockdown of target transcript with lower off-target effects (siRNA) • Medium potency targeting of multiple transcripts with shared seed sequence (miRNA) • Rapid and highly efficient production of nonisogenic or isogenic stable cell lines Modification options • Downregulation (knockdown) • Integration (knock-in) Ease of use • Predesigned constructs available for transfection in cell culture • Easy vector construction, transfection, and clone identification Type of recognition RNA–RNA DNA–DNA Issues in recognition • Seed region could play a role • Multiple integration sites may be targeted by PhiC31 in the Jump-In ™ Fast System • FRT site (34 bp) in genome is needed for gene integration; this is provided in the Flp-In ™ cell line Cell type • Mammalian • Mammalian Areas of application • Gene function • Gene-based therapeutics • Gene modulation • Pathway analysis • Systems biology • Protein production • Antibody production Limitations • Only for mRNA cleavage; degradation of mRNA is transient • Only for transient inhibition of translation (miRNA) • Only for integrations Off-target effects Moderate to high Low Multiplexing Capable No