59 Life Technologies
™
| Genome editing
Figure 17. Workflow for Ion PGM
™
sequencing to detect and confirm editing events. The 400-bp amplicons used for the
GeneArt
®
Genomic Cleavage Detection Kit and TaqMan
®
SNP Genotyping Assays served as the template for generating
a 200-bp amplicon for Ion PGM
™
sequencing. A library was prepared from the amplicon using the Ion Plus Fragment
Library Kit and Ion Xpress
™
Barcode Adapters. Template was then prepared from the library using the Ion OneTouch
™
2
System and Ion OneTouch
™
200 Template Kit v2 DL. The library was then sequenced using the Ion PGM
™
200 Sequenceing
Kit and the Ion PGM
™
System. The data were analyzed using software such as Integrative Genomics Viewer (IGV).
Amplified region
of genome
Template preparation
Sequencing analysis
(e.g., IGV)
Ion Torrent
™
sequencing
using Ion PGM
™
System
Library preparation
